超薄贴面与常规贴面临床对比研究

目的：比较超薄贴面与常规贴面的临床效果,为超薄贴面的临床应用提供依据。方法选取超薄贴面20例与常规贴面修复患者45例,基牙分别为72、120颗,3、6、12、24、36个月后随访,依据改良美国公共卫生署贴面评价分类标准进行临床效果对比研究,统计留存率和成功率。结果经过3年观察,超薄贴面和常规贴面在继发龋、边缘适合性、边缘变色、颜色适合性和结构形态方面均有较好的临床表现,留存率及成功率均大于90%,经连续性校正卡方检验差异均无统计学意义（P>0.05）。结论超薄贴面可以获得与常规贴面相近的临床效果。     

Cytologic Observations on Trypsinized Cells Obtained from Human Endometrial Epithelium in Tissue Culture

Abstract

Although epithelial cells have been successfully grown from tissue culture of endometrial explants, they survived for only a few weeks before fibroblasts overgrew and eliminated the epithelium. We describe a method for growing endometrial epithelium from menstrual blood and passing them using 0.25% trypsin in EDTA; cells survived in vitro for 24 weeks. The morphology of the processed cells differed from that observed in vitro: trypsinized cells were rounded with long surface processes and microvilli; nuclei were irregular and contained a macronucleus; the cytoplasm appeared very granular. Immunocytochemistry revealed 90–95% positivity for anti-keratin; EM confirmed the light microscopic findings. Nuclei were large and irregular with a macronucleus and cytoplasmic organelles. The most striking feature of these cells was the abundance of rough endoplasmic reticulum, surface projections, and microvilli.

Endometrial epithelium undergoing trypsinization is not irreversibly damaged and retains its epithelial characteristics although it may be mechanically disrupted as a tissue and separated into single cells or small groups of cells. (The J Hislotechnol 10:233, 1987.)     

Utility of Ki‐67, p53, Bcl‐2, and Cox‐2 biomarkers for low‐grade endometrial cancer and disordered proliferative/benign hyperplastic endometrium by imprint cytology

In this report, the authors examined the characteristic features of morphology and molecular biology of Ki‐67, p53, Bcl‐2, and cyclooxygenase‐2 (Cox‐2) immunocytochemistry in low‐grade endometrioid endometrial carcinoma (LG‐ENEC) and disordered proliferative (DP)/benign hyperplastic (BH) endometrium. We carried out a prospective study by collecting endometrial imprints from freshly resected uteri over a 20‐month period and finally 104 patients were evaluated with endometrial cytology. We focused on LG‐ENECs, as well as on BH endometrium and its precursor lesion, DP endometrium, firstly because of the overlapping cytomorphology of these pathologic entities and secondly because of the lack of agreement in the differential diagnosis of atypical hyperplasia from complex hyperplasia and well‐differentiated endometrial carcinoma, even in curettage specimens. Ki‐67 expression of LG‐ENEC showed predominance in comparison with DP/BH endometrium. Furthermore, high levels of Bcl‐2 (>50%) were expressed only in DP/BH endometrium. DP/BH endometrium was negative for p53 marker, except from two cases of BH endometrium. Cox‐2 expression ≥50% was found only in LG‐ENECs. Using Ki‐67, Bcl‐2, p53, and Cox‐2 markers, we managed to distinguish fully DP/BH endometrium from LG‐ENEC. Higher Ki‐67%/Bcl‐2% rate and also higher Cox‐2 expression were found in LG‐ENEC cases with FIGO stage ≥ IC, than in cases with FIGO stage < IC. The immunocytochemical findings from a combination of Ki‐67, p53, Bcl‐2, and Cox‐2, may differentiate LG‐ENEC from DP/BH endometrium with overlapping cytomorphology. Immunocytochemistry appeared to be useful also for the correlation between LG‐ENEC and FIGO stage. Diagn. Cytopathol. 2014;42: 134–142. © 2013 Wiley Periodicals, Inc.     

Recent advances in molecular gynaecological pathology

This review gives an overview of recent advances in our understanding of the molecular basis of gynaecological malignancy, including cervical, vulval, endometrial and ovarian tumours. These advances are beginning to influence clinical practice, perhaps most notably with the introduction of human papillomavirus vaccination for the prevention of anogenital neoplasia, but are likely to have broader clinical implications as further details emerge.     

对AgNOR用于宫内膜病变诊断的评价

目的寻找一种有助于对宫内膜不同性质病变进行诊断和鉴别诊断的客观指标。方法用核仁组成区银染法显示核仁组成区嗜银蛋白（ＡｇＮＯＲ），对增生期宫内膜、内膜增生过长，内膜癌等３组（６０例）不同性质病变进行观察对照并记数。结果３组间差别明显。内膜癌组细胞ＡｇＮＯＲ颗粒均数２７．０９，增生过长组ＡｇＮＯＲ颗粒均值１６．６２，增生期宫内膜ＡｇＮＯＲ颗粒均值１０．２４，各组间Ｐ〈０．０１，具有统计学意义。组间Ａｇ     

一贯煎联合薄芝糖肽、羟氯喹治疗干燥综合征合并血小板减少性紫癜血小板恢复正常1例报告

报告1例使用一贯煎联合薄芝糖肽、羟氯喹治疗干燥综合征合并血小板减少性紫癜血小板恢复正常。女,22岁;口干眼干,腮腺反复肿大,伴关节窜痛3年;PLT 18×109/L↓,MPV 16.2fL↑;IgA 3.47g/L↑,IgG 19.59g/L↑,IgM 1.41g/L↑,RF 404.8IU/mL↑,抗核抗体(ANA)(+),核颗粒型1:1000(++),抗SSA抗体(+),抗SSB抗体(-),抗Ro-52抗体(+)。一贯煎(黄芪、女贞子各20g,灵芝10g,生地30g,沙参15g,麦冬10g,枸杞子、当归各15g,白芍、石斛、郁金各10g,杜仲、菟丝子各20g,丹皮10g,川楝子15g,酸枣仁10g),1剂/d,水煎400mL,早晚口服;薄芝糖肽10mg/次,1次/d,静滴;羟氯喹,0.2g/d,2次/d,口服。连续治疗7.5月复查:PLT 136×109/L;口眼干涩症状缓解,无新发出血点。     

Pregnancy-associated glycoprotein (PAG) family localized in chorionic cells within the epitheliochorial/diffuse placenta of the alpaca (Lama pacos)

Pregnancy-associated glycoproteins (PAGs) are abundant embryo-originated products expressed in the pre-placental trophoblast and later in the post-implantational chorionic epithelium of some ungulate species. This paper describes the cellular immunolocalization of the chorionic PAG family in the epitheliochorial placenta type of the alpaca (Lama pacos—Lp), in which the PAGs were named ‘LpPAGs’. Placental Lp sections (5 μm) of different females near mid-pregnancy (150 days post coitum; dpc), advanced pregnancy (244-263 dpc) and late pregnancy (347 dpc) were used for cross-species (heterologous—ht) double fluorescent immunohistochemistry (htdF-IHC). The htdF-IHC was performed with primary rabbit polyvalent anti-porcine PAG polyclonals. The LpPAG immuno-complexes were visualized with secondary goat anti-rabbit immunoglobulins-conjugated with Alexa 488 fluorophore (green), among all nuclei of placental cells stained with propidium iodide (red). This is the first study reporting the immunolocalization of the LpPAG family identified by htdF-IHC at the feto/maternal interface during different pregnancy stages of the alpaca. The most dominant and strongest immune-positive LpPAG signals were found in the well-developed chorionic cell layer. Our htdF-IHC indicated relatively high epitope resemblance to that of the PAGs in camelids and pigs. These data increase our general knowledge of chorionic PAG localization during pregnancy-stage dependent development of the epitheliochorial diffuse placenta type in the alpaca.